Assay Reactions

The PCx is widely applicable to a large number of analytes among which antigens, antibodies and haptens, in all types of biological fluids. Up to now more than hundred scientific publications attest the wide application range of the technique. Determination of : Antigen, Antibody, Hapten.

ANTIGEN DETERMINATION

Latex particles are coated with antibodies. In the presence of antigens, agglutination occurs; the remaining number of particle decreases. The concentration of antigen is inversely proportional to free particles count.

Antigen coated
microparticles

Sample
antibody

Agglutinated microparticles

Free particles
(not agglutinated)

ANTIBODY DETERMINATION

Latex particles are coated with antigens. In the presence of antibodies and of an agglutination enhancer, agglutination occurs, the remaining number of particles decreases. The antibody concentration is inversely proportional to free particles count.

Antigen coated
microparticles

Sample
antibody

Antibody
fixed on to
particles

Agglutination
enhancer

Agglutinated
microparticles

Free
particles

HAPTEN DETERMINATION

Latex particles are coated with haptens. The reaction described for antibody here above, can be inhibited in the presence of haptens, compared to a reference agglutination, the number of free particles decreases.
The concentration of hapten will be directly proportional to the free particles count when compared to reference reaction.

Hapten coated particles

Anti-hapten specific antibody

Agglutination enhancer

Agglutinated microparticles

Inhibition of agglutination with sample hapten

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